High-Throughput Robotics Core Facility
This core utilizes
sophisticated equipment to provide investigators with the services of
library fractionation, replication of fractionated plasmids, bacterial
artificial chromosomes and cosmid libraries, production of arrays, preparation
of filter arrays, preparation of arrayed libraries (both custom or generic),
nitrocellulose or nylon membranes that are screened by hybridization to
identify clones of interest, clone retrieval, plasmid mini-preps, PCR
reactions, restriction digests, and agarose cell analyses. Robotic equipment
includes the Biomech 2000 (Beckman), pipetting workstations, a high-dose
density replication system (Beckman), a computerized Biomech workstation,
a 96-well UV-visible spectrophotometer (Molecular Devices), a 96-well
plate reader (Molecular Devices), an integrated electronic control system
for the instrumentation, a 96-well scintillation counter (Wallace), a
96-well cell harvester (Wallace), a 96-well PCR machine (Perkin Elmer),
software for integrated control of the robotics workstations and, finally,
a Web-based video broadcast and paging system. Dr.
James Metherall directs this core facility.
View Core Facility
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