DNA/Peptide Resource Core Facility

The DNA/Peptide Facility provides oligonucleotide synthesis, peptide synthesis and protein sequencing for researchers at the University of Utah. Routine and specialty DNA and RNA syntheses are provided at synthetic scales from 40 nmole to 10 umole. Dye or biotin labeling, phosphorylation, and phosphorthioate “antisense” synthesis are among specialty services performed. Incorporation of user-synthesized amidites into oligonucleotides is available as a routine service. N-terminal protein sequencing is available on either liquid samples or proteins transferred to pvdf membranes. Protein digests performed in user laboratories can be separated by HPLC in the Core Facility for N-terminal sequencing of isolated peptides. Routine and specialty peptide syntheses are performed at 50 umole through 250 umole scales. Biotinylated, dye-labeled, phosphorylated peptides, and peptides with D-amino acids can be produced. The core director is Dr. Robert Schackmann.

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